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Team TFS
Team TFS
Laboratorio Arbitral Agroalimentario, Ministerio de Medio ambiente y Medio Rural y Marino, Ctra. Coruña Km 10,700, 28023 Madrid, Spain
Journal of Agricultural and Food Chemistry (2010), V58, pp722–728, doi:10.1021/jf9029095
Simultaneous Stable Carbon Isotopic Analysis of Wine Glycerol and Ethanol by Liquid Chromatography Coupled to Isotope Ratio Mass Spectrometry
Ana I. Cabañero, Jose L. Recio and Mercedes Rupérez
A novel procedure was established for the simultaneous characterization of wine glycerol and ethanol 13C/12C isotope ratio, using liquid chromatography/isotope ratio mass spectrometry (LC-IRMS). Several parameters influencing separation of glycerol and ethanol from wine matrix were optimized. Results obtained for 35 Spanish samples exposed no significant differences and very strong correlations (r = 0.99) between the glycerol 13C/12C ratios obtained by an alternative method (gas chromatography/isotope ratio mass spectrometry) and the proposed new methodology, and between the ethanol 13C/12C ratios obtained by the official method (elemental analyzer/isotope ratio mass spectrometry) and the proposed new methodology. The accuracy of the proposed method varied from 0.01 to 0.19 permil, and the analytical precision was better than 0.25 permil. The new developed LC-IRMS method it is the first isotopic method that allows 13C/12C determination of both analytes in the same run directly from a liquid sample with no previous glycerol or ethanol isolation, overcoming technical difficulties associated with complex sample treatment and improving in terms of simplicity and speed.
  • IRMS
  • HPLC
  • Carbon isotopes
  • Ethanol
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