Leaver N, Chihoho B, Welchman H, Robinson S.
Application Note 518
The HRAM analysis using the Exactive mass spectrometer demonstrates SRM comparable specificity, dynamic range, LOQ and precision in whole blood matrix. There is good correlation between SRM and HRAM results for the immunosuppressant drugs monitored.
The precision of HRAM LC-MS analysis meets current consensus guidelines and has acceptable performance to be used as a candidate clinical research method following further evaluation. All the method development time for this application was associated with the sample preparation and chromatography conditions. The mass analysis method was established in less than 5 minutes since there is no requirement to tune SRM transitions, collision energies or transfer lens voltages.
Royal Brompton & Harefield NHS Foundation Trust, Heart Science Centre, Harefield Hospital, Harefield, UK
United States
