Wang J, Hudson JC, Conaway MP, Huang Y, Huhmer A.
ASMS 2013 Poster
Metabolomics is the comprehensive study of endogenous small molecule metabolites
in biological samples. In the pharmaceutical context, understanding changes in the
metabolome due to responses of potential drug candidates could accelerate drug
discovery. Challenges in the analysis arise from the complexity or chemical diversity of
the metabolites and the large amount of chemical background in the low mass range.
In this study we investigate capillary electrophoresis (CE) and couple this separation
technique to a fast, high resolution/accurate mass (HRAM) mass spectrometer for the
analysis of charged species in plasma and urine. Plasma and urine samples are
processed with automated, label free, differential analysis software (Thermo
Scientific™ SIEVE™ 2.1 software) to extract the maximum number of significant
components present.
Thermo Fisher Scientific
United States
