Application Note 20754: This application note demonstrates the analysis of fluorescently labeled N-glycans released from proteins (bovine fetuin) and separated by hydrophilic interaction liquid chromatography (HILIC) / weak anion exchange mixed-mode chromatography. The separation is carried out with a 1.9 μm Thermo Scientific™ GlycanPac™ AXH-1 column using fluorescence detection. The method exhibits excellent separation based on charge, size, and polarity. This phase also provides a unique tool for quantifying glycans based on charge.
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