Poster Note: Purpose: Develop a chromatographic solution for high resolution separation and structural characterization of native and fluorescently labeled N-linked glycans released from various proteins using a novel mixed-mode column combined with fluorescence or mass spectrometer detection.
Methods: Native and fluorescently labeled glycans from various proteins were separated on the Thermo Scientific™ GlycanPac™ AXH-1 column, and analyzed with Thermo Scientific™ Dionex™ UltiMate™3000 FLD3400RS fluorescence detector coupled to a Thermo Scientific™ Q Exactive™mass spectrometer.
Results: A high-performance, silica-based UHPLC column (GlycanPac AXH-1) coupled to Q Exactive mass spectrometer or fluorescence detector enabled simultaneous separation of glycans by charge, size, and polarity. The bioinformatics tool allowed for a highthroughput analysis for biologically relevant glycans, either labeled or native.
United States
