The form of the mineral can affect the rate of absorption, so various counterions are included in the formula. The analysis of mineral supplements provides an interesting analytical challenge in that both the anions and cations are functional ingredients that require determination. In the past, separate assays for anions and cations would be used. Advances in multi-mode chromatography permit
both anions and cations to be resolved in one run using a single HPLC column and a universal HPLC detector.
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