This application note demonstrates the separation of 2AB-labeled N-linked glycans released from proteins (bovine fetuin) by reversed phase (RP) / weak anion exchange (WAX) mixed-mode chromatography. The separation is carried out with both 1.9 µm and 3 µm Thermo Scientific™ GlycanPac™ AXR-1 columns using fluorescence detection. The method exhibits an excellent separation based on charge, isomeric structure and size.
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