Separation of 2AB Labeled N-Glycans from Bovine Fetuin on a Novel Mixed-Mode Stationary Phase

[steve-day]

Demonstrate the analysis of fluorescently labeled N-glycans released from proteins (bovine fetuin) and separated by hydrophilic interaction liquid chromatography (HILIC) / weak anion exchange mixed-mode chromatography. The separation is carried out with a 1.9 µm Thermo Scientific™ GlycanPac™ AXH-1 column using fluorescence detection.