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Separation of 2AA-Labeled N-Glycans from Human IgG on a High Resolution Mixed-Mode Column

Team TFS
Team TFS
This application note demonstrates the separation of 2AA-labeled N-linked glycans released from human immunoglobulin G (IgG) using a reversed phase / weak anion exchange mixed-mode column (Thermo Scientific™ GlycanPac™ AXR-1, 1.9 µm, 150 × 2.1 mm) with fluorescence detection. The method exhibits excellent separation of glycans based on isomeric structure, charge and size.
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Last update:
‎02-07-2014 11:46 AM
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