This application note demonstrates the separation of 2AA-labeled N-linked glycans released from bovine fetuin using a reversed-phase / weak anion-exchange mixed-mode column (Thermo Scientific™ GlycanPac™ AXR-1, 1.9 µm, 150 × 2.1 mm) with fluorescence detection. The method exhibits excellent separation of glycans based on charge, isomeric structure and size.
United States
