Sensitive and accurate quantitation of phosphopeptides using TMT isobaric labeling technique

[Orbitrap_SciLib]

Xiaoyue Jiang1, Ryan Bomgarden2, Joseph Brown1, Rosa Viner1, Andreas R Huhmer1

Standard mass spectrometry (MS) instrument methods used for phosphopeptide analysis in multiplexed quantitative workflows suffer from lower peptide identification rates and quantitative accuracy compared to unenriched, non-phosphorylated peptides. Using two standard multiplexed phosphoproteome samples, we developed and optimized new acquisition methods which result in more phosphopeptide identifications with less ratio distortion compared to traditional methods. We also applied these improved methods to a large-scale study of phosphorylation levels in A549 cell lines after insulin and IGF-1 treatment.


1Thermo Fisher Scientific, San Jose, CA 2Thermo Fisher Scientific, Rockford, IL