Viner R, Horn DM, Tao S, Orlando R. Scientific Poster Purpose:
Evaluate the performance of a new hybrid ion trap - Orbitrap mass spectrometer featuring a dual-pressure ion trap and an improved HCD collision cell for MS and MSn-based QUIBL quantification of N-linked glycans.
The N-linked glycans were permethylated with 13CH3I or 12CH2DI, mixed in different ratios, analyzed by MS/MSn on an LTQ Orbitrap Velos, and quantified.
Isobarically labeled glycans mixed in different ratios were successfully quantified using full MS, HCD, or CIS/HCD MSn spectra over 2 orders of magnitude. The maximum resolution of the Orbitrap mass spectrometer, 100000 at m/z 400, was required to resolve and quantify the 13CH3 and 12CH2D species.