on 06-12-201802:57 PM - edited on 10-15-202108:40 AM by Closed Account
Tabiwang N. Arrey1, Elena Sokol2, Angela Criscuolo1, Claire Dauly3, Alexander Harder1, ASMS 2018 Purpose: Develop a reproducible workflow for deep plasma profiling of lipids including sample
extraction and LC-MS parameters on a Thermo Scientific™ Q Exactive™ HF-X hybrid quadrupole Orbitrap™ mass spectrometer (MS) with acquisition in positive and negative ionization modes in a single injection.
Methods: Analysis of lipid extracts from human plasma. An isotopically labeled lipid standard mixture (SPLASH™ Lipidomix™ Mass Spec Standard, Avanti Polar Lipids) was spiked into each sample prior to lipid extraction. A full workflow optimization was performed including the determination of optimal plasma volume, MS source conditions and detection parameters. Thermo Scientific™ LipidSearch™ software was used for lipid identification and relative quantification. Limits of quantification were evaluated for all detected SPLASH standards using the optimized workflow.
Results: A robust and sensitive MS based workflow for plasma lipid profiling was developed,
resulting in the confident identification and relative quantification of more than 500 lipids across 13
lipid classes. Low limits of quantification were demonstrated with LOQs down to 3 ng/mL.
Thermo Fisher Scientific, 1Bremen, Germany, 2Hemel Hempstead, UK, 3Villebon-sur-Yvette, France