Mark Schroeder1, Ioanna Ntai1, SiegrunMohring2, Markus Kellmann2, Alexander Harder2, Ralf Tautenhahn1, Amanda Souza1, and Andreas Huhmer1
Purpose: To develop robust metabolomics workflows using the new Thermo Scientific™ Orbitrap Exploris™ 240 mass spectrometer.
Methods: Chemical standards and complex biological matrices were analyzed with Thermo Scientific™ Vanquish™ UHPLC system and Orbitrap Exploris 240 MS. Data were processed using Thermo Scientific™ Compound Discoverer™ software and Thermo Scientific™ TraceFinder™ software.
Results: Robust reproducible measurements were achieved over multiple days with sub-ppm mass accuracy and signal response between 5 –10% CV. Confident elemental compositions were generated from accurate mass measurements and high-resolution settings while the AcquireX intelligent acquisition workflow resulted in more unique compounds with fragmentation spectra, thus delivering high quality data and versatility for all metabolomics workflows.
1Thermo Fisher Scientific, San Jose, CA, USA, 2Thermo Fisher Scientific, Bremen, Germany