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Revolutionizing Data Independent Acquisition on q-OT-IT Tribrid Mass Spectrometers

Reputable Mentor II
Reputable Mentor II
Blank M, Huguet R, Bomgarden R, Viner R, Puri N, Reiter L, Zabrouskov V, Huhmer A.
ASMS 2015 Poster Note 64461
Purpose: Demonstrate the application of data independent acquisition (DIA) techniques for the complete quantification of a whole cell lysate using a new state of the art mass spectrometer system with improved sensitivity. Methods: Several data dependent acquisition experiments were performed and the results used to build a large spectral library on two non-small lung cancer cell lines, one which shows resistance to a tyrosine kinase inhibitor, Erlotinib. Several classicDIA experiments were performed on both current and new MS platforms and the resulting data quantified. Results: Over 5200 proteins and 44000 unique peptides were able to be quantified from the results of the DIA analysis with run to run coefficients of variation of less than 20%. Of these, nearly 1200 significantly regulated targets were identified with p-values of <0.01. A noticeable increase in quantified proteins was seen for the new q-OT-IT mass spectrometer using test HeLa cell lysate over the previous instrument, especially at lower sample loads.

Thermo Fisher Scientific and BiognoSYS AG
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Last update:
‎10-15-2021 11:34 AM
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