Blank M, Huguet R, Bomgarden R, Viner R, Puri N, Reiter L, Zabrouskov V, Huhmer A.
ASMS 2015 Poster Note 64461
Purpose: Demonstrate the application of data independent acquisition (DIA) techniques for the complete quantification of a whole cell lysate using a new state of the art mass spectrometer system with improved sensitivity.
Methods: Several data dependent acquisition experiments were performed and the results used to build a large spectral library on two non-small lung cancer cell lines, one which shows resistance to a tyrosine kinase inhibitor, Erlotinib. Several classicDIA experiments were performed on both current and new MS platforms and the resulting
data quantified.
Results: Over 5200 proteins and 44000 unique peptides were able to be quantified from the results of the DIA analysis with run to run coefficients of variation of less than 20%. Of these, nearly 1200 significantly regulated targets were identified with p-values of <0.01. A noticeable increase in quantified proteins was seen for the new q-OT-IT mass spectrometer using test HeLa cell lysate over the previous instrument, especially at lower sample loads.
Thermo Fisher Scientific and BiognoSYS AG
United States
