Yue Xuan1, Oleksandr Boychenko2, Remco Swart2, Alexander Harder1, Thomas Moehring1
ASMS 2017 Poster
Purpose: A novel high resolution MS1 based DIA method was developed to comprehensively, reproducibly, and precisely quantify proteins in complex matrices.
Methods: Instead of quantifying the peptides on MS2 intensities, MS1 scans offer potentially higher sensitivity since the peptide precursor is not fragmented into multiple fragments.
Results: Separation of the tryptic peptides in complex proteomes was performed with 1-hour total run time on the same capillary LC coupled to both the Thermo Scientific™ Q Exactive ™ HF and the Thermo Scientific™ Q Exactive™ HF-X MS systems. > 4800 protein groups and > 46,000 peptide precursors are identified and quantified on the Q Exactive HF-X system, which produces > 20% more peptide precursors than the standard Q Exactive HF system. High resolution detection removes interferences from analytes of interest, achieving accurate and precise quantitation.
1) Thermo Fisher Scientific, Bremen, Germany
2) Thermo Fisher Scientific, Germering, Germany