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Revolutionary Proteome Profiling and Quantitation without Compromising Speed, Sensitivity, and Selectivity

Reputable Mentor II
Reputable Mentor II
Yue Xuan1, Oleksandr Boychenko2, Remco Swart2, Alexander Harder1, Thomas Moehring1
ASMS 2017 Poster
Purpose: A novel high resolution MS1 based DIA method was developed to comprehensively, reproducibly, and precisely quantify proteins in complex matrices. Methods: Instead of quantifying the peptides on MS2 intensities, MS1 scans offer potentially higher sensitivity since the peptide precursor is not fragmented into multiple fragments. Results: Separation of the tryptic peptides in complex proteomes was performed with 1-hour total run time on the same capillary LC coupled to both the Thermo Scientific™ Q Exactive ™ HF and the Thermo Scientific™ Q Exactive™ HF-X MS systems. > 4800 protein groups and > 46,000 peptide precursors are identified and quantified on the Q Exactive HF-X system, which produces > 20% more peptide precursors than the standard Q Exactive HF system. High resolution detection removes interferences from analytes of interest, achieving accurate and precise quantitation.

1) Thermo Fisher Scientific, Bremen, Germany 2) Thermo Fisher Scientific, Germering, Germany
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Last update:
‎10-15-2021 11:32 AM
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