on 06-13-201803:04 AM - edited on 10-15-202108:44 AM by Closed Account
Lisa St. John-Williams1; Tuan Hai Pham2; Therese Koal2; Anastasia Kalli3; Andreas FR Huhmer3; John A. Bowden4; Stormy Koeniger5; Florence I Raynaud6; Akos Pal6; Yasmin Asad6; Catherine L. Winder7; Andrew Southam7; Mark Viant7; Warwick Dunn7; Donna O'Neill7; Jerzy Adamski8; Tong Shen9; Luiz Valdiviez9; Oliver Fiehn10; Gregory Byram10; Rupasri Mandal11; Danuta Chamot11; David Wishart11; Facundo M. Fernandez12; David A. Gaul12; Catherine G. Vasilopoulou13; Florian Meier13; Matthias Mann14; Fuad J Naser15; Gary J Patti15; Viet D Dang16; David J. Borts16; Joseph E. Lucas1; M. Arthur Moseley1; J. Will Thompson1 ASMS 2018 Targeted metabolomics has gained popularity for the ability to rapidly quantify metabolites with a high degree of confidence, albeit with inherent limits to biochemical coverage. To address this, several groups have commercialized metabolomics kits which can quantify tens or hundreds of metabolites in a single kit. One advantage of using standardized platforms is to enable consistent measurements across experiments and between laboratories, improving the overall quality of metabolomics data. In order to accomplish this task, demonstration of consistency between laboratories is imperative. We have collaborated to conduct an international ring trial of the new AbsoluteIDQ p400HR (Biocrates AG), a kit which quantifies up to 408 metabolites across 11 different metabolite classes, using the Thermo Scientific™ Q Exactive™ line of mass spectrometers.
1Duke University School of Medicine, Durham, NC; 2BIOCRATES Life Sciences AG, Innsbruck, Austria; 3Thermo Scientific, San Jose, CA; 4National Institute of Standards and Technology, Charleston, SC; 5AbbVie, North Chicago, IL; 6Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, United Kingdom; 7University of Birmingham, Birmingham, United Kingdom; 8Helmholtz-Zentrum MÃ¼nchen (CMA), Munich, Germany; 9University of California Davis, Davis; 10University of California Davis, Davis, CA; 11University of Alberta, Edmonton, AB; 12Georgia Institute of Technology, Atlanta, GA; 13Max Planck Institute of Biochemistry, Martinsried, Germany; 14Max Planck Institute of Biochemistry, Planegg-Martinsried, Germany; 15Washington University, St. Louis, St. Louis, MO; 16Iowa State University, Ames, IA