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Reliable and Deep Proteome Coverage by Gas-Phase Fractionation of Peptides with a FAIMS Pro Interface on a Modified Quadrupole Orbitrap

Reputable Mentor II
Reputable Mentor II
Julia Kraegenbring1, Tabiwang N. Arrey1, Michael W. Belford2, Satendra Prasad2, Kerstin Strupat1, Markus Kellmann1, Thomas Moehring1, and Alexander Harder1
Hupo 2019
Purpose: Demonstrate the benefit of Thermo Scientific™ FAIMS Pro™ interface for proteomic data-dependent experiments and establish an optimized method setup with the new Thermo Scientific™ Orbitrap Exploris™ 480 mass spectrometer. Methods: A commercially available tryptic HeLa digest was separated over different gradient lengths and subsequently analyzed in data-dependent acquisition mode on an Orbitrap Exploris 480 equipped with a FAIMS Pro interface. Results: The use of peptide gas-phase fractionation with the novel Orbitrap Exploris 480 and optimized method setup increases protein identification rate by up to 15% and can be achieved with both nano and capillary flow conditions.

1 Thermo Fisher Scientific, 28199 Bremen, Germany 2 Thermo Fisher Scientific, San Jose, CA 95134
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Last update:
‎10-15-2021 11:30 AM
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