Julia Kraegenbring1, Tabiwang N. Arrey1, Michael W. Belford2, Satendra Prasad2, Kerstin Strupat1, Markus Kellmann1, Thomas Moehring1, and Alexander Harder1
Hupo 2019
Purpose: Demonstrate the benefit of Thermo Scientific™ FAIMS Pro™ interface for proteomic data-dependent experiments and establish an optimized method setup with the new Thermo Scientific™ Orbitrap Exploris™ 480 mass spectrometer.
Methods: A commercially available tryptic HeLa digest was separated over different gradient lengths and subsequently analyzed in data-dependent acquisition mode on an Orbitrap Exploris 480 equipped with a FAIMS Pro interface.
Results: The use of peptide gas-phase fractionation with the novel Orbitrap Exploris 480 and optimized method setup increases protein identification rate by up to 15% and can be achieved with both nano and capillary flow conditions.
1 Thermo Fisher Scientific, 28199 Bremen, Germany
2 Thermo Fisher Scientific, San Jose, CA 95134
United States
