Tabiwang N. Arrey,1 Rosa Viner,2 Ryan D. Bomgarden,3 Eugen Damoc,1 Markus Kellmann,1 Thomas Moehring,1 and Alexander Harder1
ASMS 2017 Poster
Purpose: To benchmark a new hybrid quadrupole-Orbitrap™ mass spectrometer for relative quantification.
Methods: HeLa cell lysate was labeled with Thermo Scientific™ TMT11plex™ reagents. Aliquots from all 11 channels were mixed in equimolar ratios and analyzed on the novel Thermo Scientific™ Q Exactive™ HF-X mass spectrometer.
Results: Up to 18% more peptidesproteins were identified and quantified in a relative quantitative study using TMT11plex compared to the predecessor Thermo Scientific™ Q Exactive™ HF mass spectrometer.
1 Thermo Fisher Scientific (Bremen) GmbH, Hanna-Kunath-Str. 11, 28199 Bremen
2 Thermo Fisher Scientific, 355 River Oaks Parkway, San Jose, CA 95134
3 Thermo Fisher Scientific, 3747 N. Meridian Road, Rockford, IL 61101
United States
