on 07-09-201207:46 AM - edited on 10-15-202111:00 AM by AnalyteGuru
Viner R, Scigelova M, Zeller M, Oppermann M, Moehring T, Zabrouskov V. Application Note 566 In this note we evaluated the impact of the technological advances of hybrid instruments on overall experimental outcome in terms of protein/peptide identification and quantitation. The number of peptides and proteins identified with the new Orbitrap Elite™ instrument were compared to those produced by a Thermo Scientific LTQ Orbitrap Velos hybrid ion trap-Orbitrap MS. In addition, a detailed assessment of the quantitative performance of the Orbitrap Elite instrument for the analysis of TMT®-labeled samples was carried out. The focus was on assessing the percentage of quantifiable peptides, the quantitative precision, and the quantitative accuracy.