on 09-23-201905:34 AM - edited on 11-09-202103:48 AM by usermigration2
Rapid and precise δ13C measurement of dissolved inorganic carbon in natural waters using liquid chromatography coupled to an isotope-ratio mass spectrometer Jay A. Brandes Skidaway Institute of Oceanography, Savannah, GA, USA Limnology and Oceanography: Methods (2009), V7 (11), doi: 10.4319/lom.2009.7.730 The measurement of the carbon isotopic composition (delta13C) of dissolved inorganic carbon (DIC) in natural systems has provided a tool for examining a variety of processes, from net primary production to anthropogenic signatures of CO2 uptake in the oceans. Over the past decade, new delta13C-DIC methods have steadily decreased the sample analysis time and volumes for this measurement. The development of a new interface capable of inline acidification and extraction of CO2 from a liquid stream (LC-Isolink™) provides breakthroughs in sample size, ease of sample handling, and speed of analyses. Typical sample size injections of 25 microL provide precision and accuracy of 0.04‰, comparable to that obtained by off-line extractions/dual inlet analyses on much larger samples.