In this work, sialic acids are determined in five representative glycoproteins by acid hydrolysis followed by HPAE-PAD. Sialic acid determination by HPAE-PAD using the Thermo Scientific™ Dionex™ CarboPac PA20 Fast Sialic Acid column is specific and direct, eliminating the need for sample derivatization after hydrolysis. The use of a disposable gold on polytetrafluoroethylene (PTFE) working electrode simplifies system maintenance while providing consistent response with a lifetime of four weeks. The rapid gradient method discussed separates Neu5Ac and Neu5Gc in ❤️ min with a total analysis time of 4.5 min. By using the Dionex CarboPac™ PA20 Fast Sialic Acid column, total analysis time is reduced, per sample eluent consumption and waste generation are reduced, and sample throughput is improved.