Stratton T, Huang Y, Pihakari K, Acworth I, Weber M.
ASMS 2012 Poster
Purpose: Demonstrate the utility of online oxidative synthesis as a tool for stability screening.
Methods: Study compounds were subjected to variable potentials in a synthesis cell in-line with an HPLC system. Potentials from -1100 to +1100 mV were applied and any products were subsequently separated by HPLC for analysis by HRAM MS. Potential vs stability analysis was performed by automatic processing of the results using a relative quan/qual software.
Results: A comparison of the results of the online synthesis system and a standard microsomal stability assay showed good correlation for compounds where oxidative enzymatic processes were the major determinate of stability.
Thermo Fisher Scientific
United States
