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Reputable Mentor II
Reputable Mentor II
Mick Greer1, Daniel Lopez-Ferrer2, Romain Huguet2, Peter Verhaert3, Greg Foster4, Vlad Zabrouskov2, Andreas Huhmer2, Peter Raus5, Ken Durbin6, Joe Greer6, Ryan Fellers6, Rich Leduc6
ASMS 2020
Purpose: Apply newly developed Top-down Label Free Quantitation (LFQ).to measure proteoform level changes between age groups from human tear fluid. Methods: Tear fluid was collected using simple non-invasive Schirmerstest strips. Samples were then submitted to Top-down LC/MS analysis with (Ultraviolet Photodissociationat 213 nm) UVPD enabled fragmentation. Resulting data was analyzed via ProSightPD4.0 (beta) for determination of quantitative changes in human tear proteoforms associated with patient age. Results: Due to numerous crosslinked species observed in tear fluid, UVPD was selected for fragmentation of these proteoforms. A significant portion of the identified proteoforms were truncated species not known in UniProt, therefore Top-down MS with ProSightPDSubsequence analysis is the preferred method to accurately identify these species.

1 Thermo Fisher Scientific, Austin, TX, 2 Thermo Fisher Scientific, SanJose, CA, 3 ProteoFormiX, Beerse, Belgium, 4 Thermo Fisher Scientific, South San Francisco, CA; 5 Vrije Universiteit Brussel, Brussels, Belgium; 6 Proteinaceous, Evanston, IL.
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Last update:
‎10-15-2021 06:08 AM
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