Qu Z, Meng F, Bomgarden RD, Rogers, Viner RI, Li J, Cheng J, Cui J, Sun GY, Gu Z.
ASMS 2012 Poster
• iodoTMT reagent could specifically label S-nitrosylated proteins.
• Anti-TMT resin successfully enriches iodoTMT-labeled peptides.
• Both S-nitrosylated proteins and modified cysteine sites could be indentified by iodoTMT switch labeling.
• iodoTMT switch assay gains high output. 79 and 65 unique Snitrosylated proteins were detected and quantified from in vitro and invivo studies, respectively.
• iodoTMT switch assay is able to profile global S-nitrosylated proteins and compare protein S-nitrosylation levels under different conditions as well. Therefore, this is an effective proteomic approach for quantitative analysis of protein S-nitrosylation.
Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO, USA.
United States
