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Reputable Mentor II
Reputable Mentor II
Qu Z, Meng F, Bomgarden RD, Rogers, Viner RI, Li J, Cheng J, Cui J, Sun GY, Gu Z.
ASMS 2012 Poster
• iodoTMT reagent could specifically label S-nitrosylated proteins. • Anti-TMT resin successfully enriches iodoTMT-labeled peptides. • Both S-nitrosylated proteins and modified cysteine sites could be indentified by iodoTMT switch labeling. • iodoTMT switch assay gains high output. 79 and 65 unique Snitrosylated proteins were detected and quantified from in vitro and invivo studies, respectively. • iodoTMT switch assay is able to profile global S-nitrosylated proteins and compare protein S-nitrosylation levels under different conditions as well. Therefore, this is an effective proteomic approach for quantitative analysis of protein S-nitrosylation.

Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO, USA.
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Last update:
‎10-15-2021 11:01 AM
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