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Quantitative Proteomics Analysis of Protein S-Nitrosylation using Sixplex iodoTMT

Reputable Mentor II
Reputable Mentor II
Qu Z, Meng F, Bomgarden RD, Rogers, Viner RI, Li J, Cheng J, Cui J, Sun GY, Gu Z.
ASMS 2012 Poster
• iodoTMT reagent could specifically label S-nitrosylated proteins. • Anti-TMT resin successfully enriches iodoTMT-labeled peptides. • Both S-nitrosylated proteins and modified cysteine sites could be indentified by iodoTMT switch labeling. • iodoTMT switch assay gains high output. 79 and 65 unique Snitrosylated proteins were detected and quantified from in vitro and invivo studies, respectively. • iodoTMT switch assay is able to profile global S-nitrosylated proteins and compare protein S-nitrosylation levels under different conditions as well. Therefore, this is an effective proteomic approach for quantitative analysis of protein S-nitrosylation.

Department of Pathology and Anatomical Sciences, University of Missouri, Columbia, MO, USA.
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Last update:
‎10-15-2021 11:01 AM
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