on 06-19-202003:15 PM - edited on 10-15-202111:45 AM by Closed Account
Bhavin Patel1, Penny Jensen1, Aaron S. Gajadhar2,Sebastien Gallien3,4, Andreas Huhmer2, Daniel Lopez-Ferrer2, Kay Opperman2, John C. Rogers1 ASMS 2020 Purpose: To assess SureQuantacquisition methods for multiplex quantitation of Ras and AKT signaling pathways.
Methods: Signaling proteins from three different cancer cell lines were quantified with and without IP enrichment using a multiplexed PRM and SureQuantinternal standard (IS)-triggered method, respectively.
Results: The SureQuantIP enrichment method enabled detection of lower abundant signaling protein targets but one is limited to the targets where an IP-verified antibody is available. The SureQuantacquisitionrequires an internal standard peptide for triggeringmethod and enabled quantitation of a majority oftargets without enrichment; however, some of the lower abundant signaling protein targets were not detected.