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Scientific Advocate II
Scientific Advocate II
1,25-dihydroxyvitamin D (1,25D) tests are important in conducting clinical research in chronic renal failure and hypoparathyroidism. Circulating 1,25D levels are a thousand-fold less than 25-hydroxyvitamin D levels, making it a challenging test that benefits from
immunoaffinity purification prior to analysis with liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). In this work, both 1,25D2 and 1,25D3 were extracted from human plasma using immunoaffinity extraction and quantified with LC-MS/MS.
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Last update:
‎07-27-2015 08:17 AM
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