on 11-18-201403:19 AM - edited on 10-15-202108:36 AM by AnalyteGuru
Kozak M, Van Natta K, Sheng S PN63783 Purpose: To develop an HPLC-MS method for the forensic toxicological analysis of the three Schedule I cathinones: MDPV, methylone and mephedrone, as well as other substituted cathinones: methedrone, ethylone, butylone and naphyrone in urine with limits of quantitation (LOQs) of 0.5 ng/mL.
Methods: Liquid/liquid extraction followed by HPLC/MS/MS analysis on a Thermo Scientific™ Q Exactive™ benchtop Orbitrap mass spectrometer.
Results: We achieved LOQs of 0.5 ng/mL with good reproducibility and accuracy for MDPV, mephedrone, methylone, methylone, ethylone and butylone. Naphyrone showed more variability and is considered qualitative using this method.