Dreyer M, Second T.
• Using High Resolution/Accurate Mass SIM analysis, Cholesterol-D6 is
linear from 50 to 50000 fmol on-column in the presence of high levels of
Cholesterol. It is possible that the LOD is lower, but standards at 1 and
5 fmol/ul were not included.
• Alternate forms of Cholesterol can also be identified in the sample using
Full Scan analysis (slide 27).
• The Q Exactive can clearly identify the difference between 13C and 15N
labeled molecules (slide 11).
• There is an effect of high levels of Cholesterol on the analysis of high
levels of Cholesertol-D6 (slide 27), but the curve remains linear over
four orders of magnitude and the LOQ is 50 fmol on-column, possibly
Thermo Fisher Scientific