Quantitation of 25-Hydroxyvitamin D3 and 25-Hydroxyvitamin D2 in Plasma for Clinical Research Using an Affordable High-Resolution Mass Spectrometer

[Orbitrap_SciLib]

Mindy Gao, Marta Kozak.
Application Note 634
Goal To evaluate the performance of the Thermo Scientific™ Q Exactive™ Focus high-resolution Orbitrap mass spectrometer as a quantitative platform for HPLC-MS analysis of 25-hydroxyvitamin D metabolites in human plasma for clinical research. Application Benefits • Simple sample preparation • Short analysis time • High selectivity • Limited matrix effects • Accurate chromatographic peak integration due to very low background • Method performance meets clinical research requirements • Method accuracy proved with NIST calibrators


Thermo Fisher Scientific