Pushing the Limits of Bottom-Up Proteomics with State-Of-The-Art Capillary UHPLC and Orbitrap Mass Spectrometry for Reproducible Quantitation of Proteomes

[Orbitrap_SciLib]

Daniel Lopez-Ferrer1, Michael Blank1, Stephan Meding2, Aran Paulus1, Romain Huguet1, Remco Swart2, Andreas FR Huhmer1
Application Note 639
Describes a higher-performing quantitative proteomics workflow compared to current methods using a Thermo Scientific™ EASY-nLC™ 1200 system with a Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer with a 75 cm long, 75 μm i.d. capillary column using both 2 and 4 hour gradients.


1Thermo Fisher Scientific, San Jose, CA, USA 2Thermo Fisher Scientific, Germering, Germany