Pushing the Boundaries of Protein Identification, Characterization, and Quantitation Using a Novel Orbitrap-Based Tribrid MS Architecture

[Orbitrap_SciLib]

Zabrouskov V, McAllister G.
Webinar
Peptide identification rates and protein sequence coverage have historically advanced in line with improvements to the detection limits and spectral acquisition rate of the mass spectrometer. Spectral acquisition rates can be improved through extensive parallelization of the acquisition process using a novel Orbitrap mass spectrometer with a tribrid configuration of three mass analyzers. This webinar demonstrates the advantages of using a novel Orbitrap mass spectrometer to dramatically improve spectral acquisition rates through massive parallelization of the acquisition process to enhance proteome coverage and general experimental throughput. Taking the parallelization theme further, multiplexed quantitation using isobaric tags such as tandem mass tags (TMT) provides an avenue for mass spectrometry based proteome wide quantitation experiments. The novel arrangement of the three mass analyzers enables a new 'synchronous precursor scanning' MS3 method which provides a new level of sensitivity, precision and accuracy to multiplexed quantitation. Examples will be discussed ranging from fractionated colorectal cancer cell line lysates to low abundance phosphopeptide enriched samples.

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Thermo Fisher Scientific