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Team TFS
Team TFS
Purification and Gas Chromatography–Combustion–Isotope Ratio Mass Spectrometry of Aroma Compounds from Green Tea Products and Comparison to Bulk Analysis
Ariaki Murata (1), Ulrich H. Engelhardt (2), Peter Fleischmann (2), Keita Yamada (3), Naohiro Yoshida (3), Dieter Juchelka (4), Andreas Hilkert (4), Toshiyuki Ohnishi (5), Naoharu Watanabe (1) and Peter Winterhalter (2)
1) Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan, 2) Institute of Food Chemistry, Technische Universität Braunschweig, Schleinitzstrasse 20, DE-38106 Braunschweig, Germany, 3) Department of Environmental Chemistry and Engineering, Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology, Yokohama 226-8502, Japan, 4) Thermo Fisher Scientific (Bremen) GmbH, Hanna-Kunath-Strasse 11, 28199 Bremen, Germany, 5) Graduate School of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan
Journal of Agricultural and Food Chemistry (2013), V61(47), pp 11321–11325, doi: 10.1021/jf403605a
A method for carbon isotope ratio (d13C) analysis was developed for compound-specific isotope analysis of tea volatiles, and the values were compared with the d13C value from bulk isotope analyses. The d13C value of 2-phenylethanol liberated via enzymatic hydrolysis of the 2-phenylethyl ß-primeveroside standard was examined first. Isotope fractionations for 2-phenylethyl ß-primeveroside from preparative high-performance liquid chromatography (HPLC) were also analyzed. The enzymatic treatment and the preparative HPLC process did not cause carbon isotope fractionations, substantiating the strategies available for d13C analysis of volatile compounds. On the basis of the gas chromatography–combustion–isotope ratio mass spectrometry data from 2-phenylethanol, it was possible to derive the conditions for enzyme treatment and preparative HPLC of the glycoconjugates of 2-phenylethanol, (Z)-3-hexenol, and benzyl alcohol isolated from green tea leaves.
  • Gas Chromatography
  • Combustion
  • Carbon isotopes
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Last update:
‎11-09-2021 03:49 AM
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