Christopher Petucci1,2, Andrew V. Zelenin1,2, Jeffrey A. Culver1,2, Meghan Gabriel1, Ken Kirkbride3, Terri T. Christison3, and Stephen J. Gardell1,2
Ion chromatography (IC) was evaluated to demonstrate its ability to highly retain and separate polar metabolites that are poorly retained and resolved by reversed-phase chromatography. Ion chromatography was coupled with mass spectrometry (IC/MS) to quantitate a variety of polar organic acids in mouse muscle. The organic acids examined cover several metabolic pathways including glycolysis, the pentose-phosphate pathway, the TCA cycle, nucleotides, and amino acids.
1Metabolomics Core Facility, Sanford Burnham Prebys Medical Discovery Institute, 6400 Sanger Road, Orlando, FL 32827
2Southeast Center for Integrated Metabolomics (SECIM), Clinical and Translational Science Institute, 2004 Mowry Road, Gainesville, FL 32610
3Thermo Fisher Scientific, 1214 Oakmead Parkway, Sunnyvale, CA 94088
United States
