on 06-21-201702:47 AM - edited on 10-15-202111:16 AM by AnalyteGuru
Daniel Lopez-Ferrer1, Romain Huguet1, Andreas Krupke1, Chien-Hsun Chen1, Aran Paulus1, Peter Raus2, Vlad Zabrouskov1, Andreas FR Huhmer1 and Peter Verhaert3 ASMS 2017 Poster Purpose: Demonstrate the potential for profiling in very short times proteoforms in tears using a microfluidic chip capable of highly efficient electrokinetically driven separations hyphenated to mass spectrometry.
Methods: Tear proteins extracted from Schirmer tips (Clement Clarke International, Harlow UK) in an aqueous solution are directly analyzed by capillary electrophoresis (CE) via ZipChip source interface (908 Devices Inc., Boston MA) coupled to mass spectrometry in a Thermo Scientific™ Q Exactive™ HF system without further sample processing.
Results: We demonstrate that very highly reproducible electropherograms can be generated from the very little protein amount present in the few microliters of tear collected on one Schirmer strip. The peaks in the electropherograms represent over 60 proteoforms in the tear sample of one single donor.
1 Thermo Fisher Scientific, San Jose, California, USA
2 MirÃ³ Center for Oculoplastic Surgery, Geel, Belgium
3 ProteoFormiX, Beerse, Belgium.