Peake DA, Kiyonami R, Yokoi Y, Fukamachi Y, Huang Y.
ASMS 2015 Poster Note 64471
• LipidSearch software provides an automated workflow for processing high quality Orbitrap LC-MS/MS untargeted lipidomics data and enables reliable and comprehensive lipid identification and quantification.
• The high mass accuracy in both MS (120K) and MS2 (30K) obtained with the Q Exactive HF instrument allows confident lipid species identification from the highly complex human serum and plasma extracts. Almost a thousand of species are identified automatically and quantified from a single LC-MS run.
• Orbitrap data combined with LipidSearch software allows simultaneous lipid ID with high coverage and quantitation. Each lipid ID was obtained with a single high quality Orbitrap MS2 scan over 4 orders of concentration dynamic range.
• The CV of the technical replicates for a majority of the quantified lipids were typically below 15% showing the excellent reproducibility of the Q Exactive HF.
Thermo Fisher Scientific and Mitsui Knowledge Industry