Jiang X, Zhang Y, Hühmer A.
ASMS 2013 Poster Note
Purpose: The effect of temperature control on protein and peptide identifications in a
shotgun proteomics experiment is evaluated.
Methods: A tryptic HeLa digest is analyzed using a data-dependent liquid
chromatography-tandem mass spectrometry (LC/MS) method at multiple
temperatures. The total number of proteins and peptides are determined by repeated
injections at the same or variable temperatures and experimental results are
compared.
Results: In single bottom-up analysis, 40 ºC provides the most protein and peptide
identifications. In replicated analyses, combining the results from experiments
performed at multiple temperatures provides a significant increase in protein/peptide
identifications compared to the triplicate runs at a single temperature.
Thermo Fisher Scientific
United States
