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Reputable Mentor II
Reputable Mentor II
Jiang X, Zhang Y, Hühmer A.
ASMS 2013 Poster Note
Purpose: The effect of temperature control on protein and peptide identifications in a shotgun proteomics experiment is evaluated. Methods: A tryptic HeLa digest is analyzed using a data-dependent liquid chromatography-tandem mass spectrometry (LC/MS) method at multiple temperatures. The total number of proteins and peptides are determined by repeated injections at the same or variable temperatures and experimental results are compared. Results: In single bottom-up analysis, 40 ºC provides the most protein and peptide identifications. In replicated analyses, combining the results from experiments performed at multiple temperatures provides a significant increase in protein/peptide identifications compared to the triplicate runs at a single temperature.

Thermo Fisher Scientific
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‎10-15-2021 11:38 AM
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