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claudia-bouman
Team TFS
Team TFS
1) Department of Life Sciences,Anglia Ruskin University,East Road,Cambridge CB1 1PT,UK, 2) Department of Geography,College of Science,Swansea University,Swansea SA2 8PP,UK, 3) Thermo Fisher Scientific,Hanna-Ku nath Str.11,28199 Bremen,Germany
Geochimica et Cosmochimica Acta (2013), V112, pp178–191, doi: 10.1016/j.gca.2013.02.021
Position-specific measurement of oxygen isotope ratios in cellulose:Isotopic exchange during heterotrophic cellulose synthesis
John S.Waterhouse (1), Shuying Cheng (1,2), Dieter Juchelka (3), Neil J.Loader (2), Danny McCarroll (2), V.Roy Switsur (1) and Lata Gautam (1)
We describe the first reported method for the measurement of oxygen isotope ratios at each position in the glucose units of the cellulose molecule. The overall process comprises a series of synthetic organic sequences, by which a-cellulose is hydrolysed to glucose, and oxygen atoms at specific positions in the glucose molecule are removed in samples of benzoic acid for measurement of d18O. Values of d18O at specific positions in cellulose are calculated from these d18O values and the overall d18O value of the cellulose. We apply the method to determine the degree to which oxygen atoms at each position undergo isotopic exchange with water during heterotrophic cellulose synthesis, such as occurs in the cambium of trees.
  • GC-IRMS
  • O isotopes
  • Cellulose
  • Position-specific
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