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Plug-and-play analysis of the human phosphoproteome by targeted high-resolution mass spectrometry

Reputable Mentor II
Reputable Mentor II

Robert T Lawrence, Brian C Searle, Ariadna Llovet & Judit Villén
Nature Methods (2016)
Systematic approaches to studying cellular signaling require phosphoproteomic techniques that reproducibly measure the same phosphopeptides across multiple replicates, conditions, and time points. Here we present a method to mine information from large-scale, heterogeneous phosphoproteomics data sets to rapidly generate robust targeted mass spectrometry (MSMS) assays. We demonstrate the performance of our method by interrogating the IGF-1/AKT signaling pathway, showing that even rarely observed phosphorylation events can be consistently detected and precisely quantified.
Department of Genome Sciences, University of Washington, Seattle, Washington, USA

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