Robert T Lawrence, Brian C Searle, Ariadna Llovet & Judit Villén
Nature Methods (2016)
Systematic approaches to studying cellular signaling require phosphoproteomic techniques that reproducibly measure the same phosphopeptides across multiple replicates, conditions, and time points. Here we present a method to mine information from large-scale, heterogeneous phosphoproteomics data sets to rapidly generate robust targeted mass spectrometry (MSMS) assays. We demonstrate the performance of our method by interrogating the IGF-1/AKT signaling pathway, showing that even rarely observed phosphorylation events can be consistently detected and precisely quantified.
http://www.nature.com/nmeth/journal/vaop/ncurrent/full/nmeth.3811.html
Department of Genome Sciences, University of Washington, Seattle, Washington, USA
United States
