Plant Metabolomics: Tomato Metabolite Profiling and Identification Employing High Resolution LC-MS Strategies

[Orbitrap_SciLib]

Welchman H, Portwood D, Earll M, Seymour M, Baxter C, Ament Z, Woffendin G, Hornshaw M, Athanas M, Oppermann M.
Scientific Poster
Purpose: Metabolite profiling of a wild-type (WT) and of a ripening inhibited (RI) tomato variety in tomato fruits selected at 4 development stages. Methods: Metabolite profiling and fragmentation were both performed on a high resolution, high mass accuracy platform. The mass spectrometer was mass calibrated prior to starting the sequence of injections. All data was acquired using external calibration and positive ionization mode. Results: Masses were measured with high, sub-ppm to max 2 ppm accuracy, leading to identifications based on elemental composition analysis.


Thermo Fisher Scientific