Bromirski M, Scheibner O, Arrey TN, Strupat K.
Application Note 30247
The importance of Atmospheric Pressure Matrix Assisted Laser Desorption/Ionization (AP MALDI) technique for analyzing larger biological or synthetic samples is investigated on the novel single stage Orbitrap analyzer, the Thermo Scientific Exactive Plus MS. The Exactive Plus instrument allows access to mass range up to m/z 6000. The performance of this device is presented by detecting MALDI-ions produced under atmospheric pressure. Larger endogenous peptides are investigated with regard to mass resolution, isotope pattern and signal-to-noise ratio. Intact proteins are prepared with 1,5 DAN matrix for the application of In Source Decay (ISD) fragmention
of proteins; ISD fragments of medium-size proteins are interpreted. Samples are prepared using published preparation protocols for MALDI MS. Synthetic polymers such as PEGs are investigated, specifically the performance of the entire polymer distribution and the adduct ion formation are explored.
Thermo Fisher Scientific
United States
