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Peptide Quantification using Parallel Reaction Monitoring Analysis Performed on a Quadrupole‐Orbitrap Instrument

Reputable Mentor II
Reputable Mentor II
Gallien S, Duriez E, Peterman S, Demeure K, Domon B.
ASMS 2012 Poster
• The novel hybrid quadrupole‐orbitrap mass spectrometer offers unique capabilities for high resolution /accurate mass measurements, while enabling isolation of low abundance components by setting a narrow m/z selection range. • The MS/MS capabilities of this instrument provide an additional stage of selectivity by performing quantification on fragment ions. • To scale up the number of peptides targeted in one single LC‐MS/MS analysis, acquisition parameters need to be adapted to maintain acceptable cycle times. It includes the relaxation of orbitrap resolving power, and the simultaneous measurement of several peptide fragmentation spectra, which implies the selection of wider precursor m/z ranges or several precursor m/z ranges (multiplexed analysis). • The performance of the Parallel Reaction Monitoring (PRM) method was explored by a systematic evaluation of the pertinent instrumental parameters to estimate (e.g. quadrupole isolation window, orbitrap resolution) their impact on selectivity, sensitivity, and the number of peptides targeted in one LC‐MS experiment.

Luxembourg Clinical Proteomics Center
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Last update:
‎10-15-2021 06:11 AM
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