Overcoming the challenges in Data Independent Acquisition (DIA) via high resolution accurate mass Orbitrap based mass spectrometer

[Orbitrap_SciLib]

Xuan Y, Muntel J, Berger S, Huhmer A, Steen H, Moehring T.
ASMS 2015 Poster
Purpose: Utilize Thermo Scientific Q Exactive HF mass spectrometer for DIA LC-MS/MS experiments to study the urinary proteome, and demonstrate how high resolution/accurate mass spectrometry is employed to overcome DIA challenges and enable large scope of urine proteomics studies. Results: In this study, we have developed a robust DIA method for the comprehensive mapping of the urinary proteome that enables large scale urine proteomics studies with high throughput ( less than 4 days for 87 biological sample measurements) and excellent reproducibility (media of CV% is 7%).


Thermo Fisher Scientific and Boston Children's Hospital