Optimizing the Isolation Width in Orbitrap Instruments to Maximize the Number of Label-Free Quantified Peptides and Proteins

[Orbitrap_SciLib]

Carmen Paschke1; David Horn2; Tabiwang N. Arrey1; Rosa Rakownikow Jersie-Christensen1; Martin Zeller1; Romain Huguet2; Pedro Navarro1, Bernard Delanghe1
ASMS 2019 Poster
Purpose: Maximizing the number of quantified proteins in Label Free Quantification experiments. Methods: Optimizing isolation width and using improved identification methods. Results: Optimized method produces 50% more protein groups.


1Thermo Fisher Scientific, Bremen, Germany 2Thermo Fisher Scientific, San Jose, CA