Optimizing Top Down Analysis of Proteins on Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer

[Orbitrap_SciLib]

Sharma S, Mallick P, Stoyanova T, Mullen C, Weisbrod C, Canterbury J, Horn D, Zabrouskov V.
ASMS 2015 Poster Note 64439
Purpose: In this work we optimize top down analysis of intact proteins with the goal of maximizing sequence coverage on a Thermo Scientific™ Orbitrap Fusion™ Lumos™ Tribrid™ Mass Spectrometer Methods: Intact proteins were characterized using ETD High Dynamic range (ETD HD), HCD, and CID either by direct infusion into the mass spectrometer or online LC- MS Results: We demonstrate high sequence coverage of intact proteins using top-down fragmentation on an Orbitrap Fusion Lumos Mass Spectrometer


Thermo Fisher Scientific, Stanford University, and University of California