Sebastien Gallien1,2, Aaron S. Gajadhar3, Bhavin Patel4, Carmen Paschke5, Dave Horn3, Nicholas Shulman6, Bernard Delanghe5, Brendan MacLean6, Daniel Lopez-Ferrer3, Emily I. Chen2, Shannon Eliuk3, Andreas Huhmer3
International Hupo 2020
The SureQuantTM methodology has recently been implemented natively in the instrument control software of Orbitrap-based mass spectrometers. It uses spiked-in internal standards (IS) to dynamically control the acquisition process and to maximize its productivity. In addition to the resulting enhanced data quality and expanded scale of targeted experiments, the technique exhibits an improved usability and robustness, owing to its independence from time-scheduling acquisition, and therefore high potential for automation. In order to progress further towards a turnkey solution, several informatic developments have been conducted to optimize the preparation of SureQuant method and the processing of generated data.
1Thermo Fisher Scientific, Paris, France; 2Thermo Fisher Scientific, Precision Medicine Science Center, Cambridge, MA, USA; 3Thermo Fisher Scientific, San Jose, CA, USA; 4Thermo Fisher Scientific, Rockford, IL, USA; 5Thermo Fisher Scientific, Bremen, Germany; 6University of Washington, Seattle, WA, USA.