Optimized DDA+ and HR-DIA workflows for standardized, reproducible, precise and robust label-free quantitation of proteomes

[Orbitrap_SciLib]

Aaron S. Gajadhar1, Oleksandr Boychenko2, Xin Zhang3, Yue Xuan4, and Andreas Huhmer
ASMS 2018
Purpose: To overcome the limitations of current label-free proteome quantitation (LFQ) protocols, we sought to develop an end-to-end, complete LFQ workflow solution for improved robustness, reproducibility, and throughput while still retaining high-performance. Methods: A configuration of high-performing, yet robust and easy-to-use components, were optimized to satisfy the analytical requirements necessary for: standardized operation; reproducibly comprehensive results; a readily scalable workflow; and quantitatively precise measurements of proteomes. Results: Complementary label-free precursor-ion based MS1 quantitation workflows — data-dependent acquisition plus (DDA+) and high-resolution DIA (HR-DIA) — allow highly sensitive and robust measurements to be collected in a cap-flow high-throughput based configuration.


Thermo Fisher Scientific, San Jose1, CA, USA; Germering, Germany2; Sunnyvale, CA3, USA; Bremen, Germany4