Optimization of Crosslinked Peptide Analysis on an Orbitrap Fusion Lumos Mass Spectrometer

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To improve identification of protein-protein interactions, MS-cleavable crosslinkers were evaluated for crosslinked peptide analysis on a high-end Tribrid mass spectrometer. Also compared were different types of fragmentation (CID, ETD) and levels of tandem mass spectrometry (MS2 and MS3).