Novel capillary-flow LC HRAM MS platform for fast targeted analysis and robust profiling of complex samples

[Orbitrap_SciLib]

Alexander Boychenko1, Peter Bults2, Martin Ruehl1, Christopher Pynn1, Mike Baynham3, Wim Decrop1, Alexander Harder4, Nico van de Merbel2, Rainer Bischoff2, Remco Swart1
ASMS 2017 Poster
Purpose: Here we present a highly robust novel capillary-flow LC-MS platform that combines the Thermo Scientific™ capillary-flow UltiMate™ 3000 RSLCnano system (capLC), the new 150 μm ID Thermo Scientific™ EASY-Spray™ column and the new Thermo Scientific™ Q Exactive™ HF-X mass spectrometer. Methods: We used typical shotgun and targeted proteomics experiments (Full-scan MS, Data-Dependent Acquisition (DDA), and Parallel-Reaction Monitoring (PRM)) to verify the performance and robustness of the novel capLC-MS platform. Results: We show that the novel capLC-MS platform is a sensitive and reliable solution for shotgun and targeted high-resolution accurate-mass (HRAM) proteomics experiments that can be used for routine proteome profiling of complex samples including bio-fluids as well as targeted high-throughput quantification.


1 Thermo Fisher Scientific, Germering. Germany 2 University of Groningen, Groningen, The Netherlands 3 Thermo Fisher Scientific, Runcorn, UK 4 Thermo Fisher Scientific, Bremen, Germany