New method filters for improved MSn acquisition for small molecule and proteomics workflows

[Orbitrap_SciLib]

Graeme McAlister, Ioanna Ntai, Rieko Kiyonami, Romain Huguet, Caroline Ding, Iman Mohtashemi, Derek Bailey, Shannon Eliuk, Vlad Zabrouskov, Seema Sharma
ASMS 2018
Purpose: We describe the new filtering capabilities of the Thermo Scientific™ Orbitrap™ method editor software, which directs the instrument towards efficiently collecting more meaningful and informative tandem mass spectrometry (MS/MS) and higher-order MSn spectra. Methods: We developed and evaluated these filters using a combination of small molecule samples (human plasma and flavonoids) and a complex Tandem Mass Tag (TMT) labeled peptide sample. Results: The AcquireX workflow guides the instrument towards efficiently collecting MSn data at exhaustive breadth and depth, while the MSn Quality Trigger informs the instrument of when it is appropriate to collect high-value and high-cost MSn spectra.


Thermo Fisher Scientific, San Jose, CA